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By John H. Hash

The seriously acclaimed laboratory average, tools in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper this present day - really a necessary book for researchers in all fields of existence sciences.

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Ishikura, and A. Osaki, Folia Microbiol. (Prague) 16, 218 (1971). 22R. C. Pittenger and E. McCoy, J. Bacteriol. 65, 56 (1953). 38 METHODS FOR THE STUDY OF ANTIBIOTICS [2] advantageous by some investigators in selecting improved antibiotic producers; others have reported poor correlation when isolates selected by this method were eventually tested in shaker flasks. In any case, the following suggestions will help avoid pitfalls: (a) If the antibiotic is not bactericidal, the colonies to be overlayered should first be replica-plated 2~ to another set of plates before flooding with the test organism.

Many E. coli strains may be obtained from the Coli Genetic Stock Center, Yale University (Curator, Dr. Barbara Bachman), and S. typhimurium can be obtained from the Salmonella Stock Center, University of Alberta, Calgary (Curator, Dr. Kenneth Sanderson). The authors of referenced manuscripts are the best source of strains which have been described in publication. 2S. " University Park Press, Baltimore, Maryland, 1971. "The Problems of Drug-Resistant Pathogenic Bacteria" (E. L. Dulaney and A. ), Ann.

F. Stauffer, Amer. J. Bot. 41, 1 (1954). 34 METHODS FOR THE STUDY OF ANTIBIOTICS T A B L E II PROCEDURE FOR N I T R O G E N M U S T A R D Spore suspension Flask no. 0 [2] ( H N 2 ) ~ TREATMENT 2% NaHCO3 (ml) 1% HN2 (ml) HN2 Conc. 01 0. 1 30 30 30 30 30 • HN2 = methyl-bis(fl-chloroethyl)amine. platings on suitable media are then completed. 1~o H N 2 levels frequently produce a reasonably satisfactory balance between number of survivors and improved mutants in Streptomyces. Exposure time or H N 2 concentration m a y be increased if necessary; killing of spores is usually rather rapid, and an increase in morphological variants is frequently observed.

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