By Nitar Nwe
These days biopolymers akin to agar, agarose, alginate, carageenan, cellulose, chitin, chitosan, collagen, hyaluronic acid, gelatin, glucan, starch, DNA, RNA and protein were made from laboratory to commercial scale. The physico-chemical homes of those biopolymers resembling chemical compositions, solubility, molecular weight and viscosity are of significant significance at the coaching of micro- and nanomaterials akin to powder, resolution, hydrogel, micro- and nano-sized pores in membrane, micro- and nano-fiber, macro- and micro-beads, nano-particles, and micro- and nano-structured scaffold. those micro and nano-materials were utilized in a number of sectors corresponding to agriculture, nutrients, medication, and so forth. This monograph will handle the resource and construction equipment of biopolymers, houses of biopolymers, training of micro- and nano-materials utilizing biopolymers, characterization of micro- and nano-biomaterials and alertness of micro- and nano-biomaterials
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Additional info for Biopolymers based micro-and nano-materials
Forms Applications 68 Hydrogel membrane Wound dressing 69 Electrode Nano powder 70–72 Food industries (noodles, sweeteners, Powder dextrins, monosodium glutamate (MSG), modified starch, crackers, cakes, syrups, desserts, puddings, creams, bread, cookies, varies fresh snacks); paper industries (coatings and sizing in paper); textile and carpets industries (binders, adhesives, absorbents); brewing industries; glue industries; cosmetics industries; medicine pharmaceuticals industries; ceramics and construction (spray concrete) 73 Nanoscale biofiller Nano crystals 74 Tissue engineering Scaffold Table 6-8 Various applications of DNA.
The weight of water absorbed in material (Wa) was calculated as a fraction of dry weight of the material as shown in Equation (5-2). The values were expressed as the means value of three independent replicates [49, 53]. org/about-asme/t Characterization of Macro-, Micro- and Nano-Biomaterials 43 5-C Mechanical properties of micro- and nano-biomaterials Neutralized materials were cut into strips in wet or dry condition to prevent deformation of the samples. For the wet sample, the materials was freeze-dried after by soaking in water.
Fresh medium was provided every 1–3 days depending on the incubation period. The number of proliferated cells was counted after 5–6 days of incubation. Prior to cell counting, sample was washed 3 times with PBS buffer to remove unattached cells and FBS from the medium. org/about-asme/t Characterization of Macro-, Micro- and Nano-Biomaterials 45 to detach the cells from the sample. The cell suspension was then sheared mildly with a micropipette to detach the cells from the sample material. After detachment of all cells from the sample (confirmed by microscopic observation), the sample was removed from the cell suspension and the cell suspension was centrifuged at 1000 rpm for 5 min.