By Garcia, Lynne Shore; Isenberg, Henry D
A collaborative attempt of a hundred and fifty+ scientific microbiologists, scientific laboratory technologists, and laboratory supervisors. presents step by step protocols and outlines to let scientific microbiologists and laboratory employees group of workers to accomplish all analyses.
This identify is released by means of the yankee Society of Microbiology Press and allotted through Taylor and Francis in remainder of international territories.
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Additional info for Clinical Microbiology Procedures Handbook Volumes 1-3
Transport to laboratory immediately. Often only a few microorganisms present. Scraping should be done by ophthalmologist. Consult with physician about use of terms or the handling of the specimen (CD-RIGHT EYE, OS-LEFT EYE). Giemsa and Gram stains are frequently requested. Uncontaminated fluid Sterile tube Premature rupture of membranes >24 h Sterile container with TM; appropriate collection containers for demonstration of specific organisms, such as N. gonorrhoeae, Chlamydia, HSV, etc. 1-1). Venereal disease, postpartum infection Genital tractfemale Amniotic fluid Cervix (endocervix) Wipe cervix clean of vaginal secretion and mucus.
Anaerobes Specimen for anaerobes not received in appropriate container Anaerobic cultures requested on autopsy material; bronch wash; decubitus ulcer material (not punch biopsy of tissue beneath eschar); drain; drain site; environment; exudate; feces; gastric washing (other than newborn); midstream or catheterized urine; mouth, nose, or prostatic secretions; sputum material on swabs from ileostomy or colostomy; fistual or intestinal contents; throat or vaginal secretions Aerobic bacteriology Gram stain for Neisseria gonorrhoeae on specimens from cervix, vagina, and anal crypts Specimens for GC and/or Chlamydia culture received in Gen Probe transport medium Respiratory culture requested on throat specimens of patients older than 10 yr Sputum specimen with <25 WBC, >10 epithelial cells/lpf Sputum with or without WBC and numerous and varying tinctorial bacterial morphotypes for Legionella DFA Procedure Notify physician or nurse in charge.
Consider venereal, fungal, anaerobic, and AFB infection. Ulcerations should be checked for syphilis, soft chancre, or genital herpes. Yeast common. For GC, cervical specimen is preferred. Wet mount is for yeast and Trichomonas, “clue cells,” Gardnerella, and organisms of vaginosis. Postsurgery Discharge Same as above Characteristic motility is seen only on warm material. Seal coverslip or capillary tube with lanolin or petrolatum. Specimen should be examined within 15 min of collection because the organism loses motility as the temperature decreases.